|Instrument setting issue||Tobi||2020-06-08|
Dear Skyline team,
I noticed the Instrument settings/ Min Max m/z applies to both fragments and precursors at the same time. Did skyline always behave like this? I find it a bit troublesome as fragment measurement ranges are nearly all the time much wider than precursor measurement ranges. If I use this setting to properly filter precursors I loose lots of fragments, and if I properly filter fragments I spend alot of time on loading sometimes thousands of nonsense precursors into the target list.
Do you have a good tip or workaround on how I can differently filter precusors/fragments when building a target list?
Looking forward to a short reply.
[Skyilne-daily 126.96.36.199, add peptide PEPTIDER++, filter for 400 to 500 mz yields only 3 fragments]