Hi Tobi,
I have considered in the past changing this to be two sets of min and max values, as you suggest. However, 1 is appropriate for SRM and most of the time when people do DIA, the min and max precursor range is controlled by a prespecified isolation scheme, and DDA with MS1-only extraction also only requires one min and max for controlling the precursor m/z values.
So, the cases where this is still less than ideal might be summarized as:
- PRM
- DIA with a "Use results" isolation scheme
- DDA with fragment ion extraction (unusual, but people do this and we now support it with an option in the MS/MS filtering Isolation scheme dropdown list)
Are you using one of these methods, or something else that requires what you are asking for.
In these cases, we don't yet have an answer for you, if you want to limit both precursors and products to different ranges. Maybe we should add it to our list for Skyline 20.2. But, yes, this has always been the case, and hardly anyone has complained about it.
Can you describe your exact experimental setup? PRM seems the most validly problematic to me. If you are doing either of the other two, I would suggest using a prespecified isolation scheme for DIA and we are just less interested in the fragment ions from DDA case.
Thanks for your feedback.
--Brendan