Failed Importing .raw Waters MSe Data

Failed Importing .raw Waters MSe Data mcbagley  2019-11-07

Hello Skyline team,

I have spent the past while searching previous posts for my particular error and could not find it. I am trying to upload MSe proteomics data acquired on a Waters G2. When I try to import the .raw files I get the attached error. Unfortunately, these data files cannot be shared. The error occurs for any attempted import of these .raw files.

I'm using Window 10 Pro with 64-bit operating system. I'm using Skyline (64-bit)

I do apologize if this is a trivial fix.

Thank you!

Brian Pratt responded:  2019-11-07

That's unusual. Could we see the .raw file, and the Skyline document you're using with it (use Skyline's File>Share>Complete menu item to create a file). You can upload to , or we can arrange something else if you prefer.


Brian Pratt

mcbagley responded:  2019-11-08

Thank you for your quick reply, Brian! Let us find another way to get this to you if possible. I have an example file and the Skyline file ready to send to you.

Thank you!

Brian Pratt responded:  2019-11-08

If you want to keep it private, we can use dropbox, google drive, whatever is good for you. I'll contact you off-list.

Brian Pratt responded:  2019-11-21

Got the files, thanks.

Using the current Skyline-Daily, I did have to change the Transitions>FullScan settings for MS1 from Centroided to TOF, as Skyline gave a "no centroided data in this file" warning, but otherwise had no issues loading the data.

Is the data on a network drive? That sometimes causes problems. If so, try copying to your local drive.

Let me know how it works out.