|Error building spectral library from TPP/PeptideProphet pep.xml||david schmidt||2019-11-06|
I'm trying to import search results produced by X!Tandem and further analysed by PeptideProphet using the TPP, in order to build a spectral library in Skyline. The original data was acquired using a DDA approach on a waters QTOF, centroided and lockmass-corrected in MassLynx and then converted to MzML using MSConvert. Importing the MzML as results works fine. However, when trying to build a spectral library from either the interact.pep.xml or the tandem.pep.xml file, I'm getting the error: No spectra were found in the new library (see attached).
I suspect that this may be due to the link within the search results that point to the spectra in the MzML file. A pretty similar problem was described before, but I couldn't figure out what the solution to that was:
Thank you very much for your help,