|using one peptide calibration curve to quantify another peptide||ana||2019-06-06|
Hello Skyline team,
following up on some of the items of this previous discussion:
I am still having problems figuring out how to quantify a peptide for which I don't have standards using the calibration curve generated for another peptide. I'll try to describe my workflow as well as possible (attached step by step snapshots):
I know that this is not best practice, but instead of reporting just areas, I would like to report a concentration (even if it's far off from the real value, it can be used for comparing different samples and give an idea of how much it's present). I can do this type of analyses with the software provided by our LCMS and would love to find out if I can also do it using Skyline (since I am quantifying all other peptides for which I have standards with it).
Thanks in advance!