using one peptide calibration curve to quantify another peptide | ana | 2019-06-06 13:47 | |||||||||||||||||||||||||||
Hello Skyline team, following up on some of the items of this previous discussion: https://skyline.ms/announcements/home/support/thread.view?rowId=36379 I am still having problems figuring out how to quantify a peptide for which I don't have standards using the calibration curve generated for another peptide. I'll try to describe my workflow as well as possible (attached step by step snapshots):
I know that this is not best practice, but instead of reporting just areas, I would like to report a concentration (even if it's far off from the real value, it can be used for comparing different samples and give an idea of how much it's present). I can do this type of analyses with the software provided by our LCMS and would love to find out if I can also do it using Skyline (since I am quantifying all other peptides for which I have standards with it). Thanks in advance! |
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