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Can we run metabolomics data recorded from Agilent Qtof mass spectrometer using Skyline

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Can we run metabolomics data recorded from Agilent Qtof mass spectrometer using Skyline bhaswatic  2019-03-08 11:38
 

Hi,
Can we run metabolomics data recorded from Agilent Qtof mass spectrometer using Skyline?
Please let me know the details about it that is how to do it as I am new to Skyline.
Thanks in advance.
Thanks and Regards,
Bhaswati
 
 
Brian Pratt responded:  2019-03-08 13:44

Absolutely!

I would suggest starting by looking at the Small Molecules section at
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorials

Best Regards,

Brian Pratt
 
bhaswatic responded:  2019-03-10 04:09

Thank you for your reply.
Actually I have 6 mass spectrometry runs recorded in Agilent QTof . Out of the 6 runs , three runs corresponds to diabetic mouse metabolites and other three runs belong non-diabetic mouse metabolites. I want to see total how many metabolites are characterised and present in total 6 mass spectrometry runs, then I would like to quantify the metabolites. Please let me know how to run the data using skyline. How to convert Agilent QTof MS/MS files to skyline files ?
Thank you for help.
Regards,
Bhaswati.
 
Brian Pratt responded:  2019-03-11 15:10

Did you get a chance to look at the tutorials I recommended?