DIA\Panorama | brown459 | 2018-08-23 12:05 | |||||||||||||||||||||
I am trying to set up a skyline method that I can use to port data to panorama so that I can share data with a collaborator. I should describe what I am attempting to do. I have a collaborator that has some samples and a control. I am trying to generate a DIA project that I can quantify the peptides found in my previous DDA searches (Using DIA to increase my coverage). Then I want to be able to share this data with my collaborator. She is interested in the relative abundances of the different proteins/peptides in the different samples. I have so far made a skyline document that has the concatenated DDA runs (from all files) and am in the process of acquiring the DIA data from those same samples. I am of the opinion that the DIA will give me better confidence and quantification overlap. I am not sure if I am setting my skyline document up wrong/or my panorama page up wrong— I would love any help! I have attached the method that I was planning on using. And am in the process of running the DIA experiment. I have already reached out to Vagisha and she mentioned that I should also post here. CJB |
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