High error rate in Skyline daily's peak picking | graceac9 | 2018-06-20 16:45 | |||||||||||||||||||||
I am doing a DIA project with oysterseed proteomic data. The protocol I have calls for checking Skyline's peak-picking accuracy. I have gone through it a few separate times and picked 100 random peptides and gave them a score of 0 (incorrect peak; no peak; incorrect peak boundaries) or a 1 (correct peak and correct boundaries). The error rates for samples were around 50%. Two other people in my lab have done similar projects within the last 1.5 years, and their error rates were around 30%. I have gone through the protocol and checked settings again and again, but my error rates are still very high. Please let me know if there is any more information I can provide on this to help resolve this issue! Thank you. Attached is my file containing my peak-picking error rates |
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