Support for isotope labeling studies

support
Support for isotope labeling studies md8  2018-06-11 03:34
 

Hi Skyline team,
I wish to know if skyline can support targeted analysis of 13C labeled metabolites. Basically if MS1 precursor along with its isotopic distribution is specified can skyline do the MS1 peak integration for unlabeled and labeled peaks? I am interested because skyline already supports MS1 and MS2 peak integration for proteomics studies and as there are very limited options available for metabolite studies in this regard, any help from your side would be appreciated.

 
 
Brian Pratt responded:  2018-06-11 08:42
Absolutely! Have a look at the "Small Molecule Quantification" tutorial at https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorials for an example.

Thanks for using Skyline!

Brian Pratt