Indispensible! Angela Jackson  2011-03-08 18:22
Being that Skyline is vendor neutral, this powerful software is becoming a necessity for all our quantitative collaborations. We regularly work with suites of peptides synthesized with differing heavy labels and Skyline’s ability to assign modifications on a peptide-specific level is indispensible. PAR data is displayed directly in the peptide list allowing for easy identification of ions with interference. Skyline also took the laborious task of uploading a protein sequence, creating a scheduled MRM transition list, optimizing the CE and generating a final MRM method of the most intense ions and made it efficient! Skyline is truly an indispensible tool for targeted proteomic applications.

Angela Jackson
Research Associate
UVic GBC Proteomics Centre
Victoria, BC, Canada