Being that Skyline is vendor neutral, this powerful software is becoming a necessity for all our quantitative collaborations. We regularly work with suites of peptides synthesized with differing heavy labels and Skyline’s ability to assign modifications on a peptide-specific level is indispensible. PAR data is displayed directly in the peptide list allowing for easy identification of ions with interference. Skyline also took the laborious task of uploading a protein sequence, creating a scheduled MRM transition list, optimizing the CE and generating a final MRM method of the most intense ions and made it efficient! Skyline is truly an indispensible tool for targeted proteomic applications.
UVic GBC Proteomics Centre
Victoria, BC, Canada