Dear Skyline team,

I am trying to create a PRM PASEF method. For this, first i measured some samples on DDA and searched the raw file in Fragpipe. I uploaded that file to create library in Skyline and used the same result files to create mobility library . Now I want to filter the peptides, I saw most of them just have precursors ions no transition ions even though I have selected p, y, b ions in the settings.

Can you please have a look and let me know if I have done something wrong.

Best,
Susmita