Exporting Skyline Method to UNIFI for Waters Xevo QTof G3

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Exporting Skyline Method to UNIFI for Waters Xevo QTof G3 philipp peslalz  2025-06-30 02:09
 

Hi all,

I’m currently working with the Waters Xevo QTof G3 in combination with UNIFI software, and I’m hoping to use Skyline to design my proteomics acquisition methods (specifically for PRM/MRM-HR workflows).

My main question is:
How can I export a method from Skyline and import it into UNIFI?

I’ve successfully exported a transition list (or isolation list) from Skyline as a .csv, but I haven’t found a way to directly import this into UNIFI as an acquisition method. Is there a workflow or tool available to help bridge this gap?

Any advice or examples from others who’ve used Skyline with UNIFI would be greatly appreciated!

Thanks in advance,
Philipp