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import MS1 data failed using Skyline (64-bit) 25.1.0.142 (7401c644b4)

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import MS1 data failed using Skyline (64-bit) 25.1.0.142 (7401c644b4) zhijun cao  2025-06-23 11:37
 

At 2:36 PM:
Failed importing results file '\prusntyfil01\home$\SU012236\Documents\project\raws\20250619_h10n3_DS\DS\Tris_02.raw'.
boost::filesystem::status: Incorrect function [system:1]: "\prusntyfil01\home$\SU012236\Documents\project\raws\20250619_h10n3_DS\DS\Tris_02.raw"
pwiz.Skyline.Model.Results.ChromCacheBuildException: Failed importing results file '\prusntyfil01\home$\SU012236\Documents\project\raws\20250619_h10n3_DS\DS\Tris_02.raw'.
boost::filesystem::status: Incorrect function [system:1]: "\prusntyfil01\home$\SU012236\Documents\project\raws\20250619_h10n3_DS\DS\Tris_02.raw" ---> System.Exception: boost::filesystem::status: Incorrect function [system:1]: "\prusntyfil01\home$\SU012236\Documents\project\raws\20250619_h10n3_DS\DS\Tris_02.raw"
at pwiz.CLI.msdata.ReaderList.read(String filename, MSData result, Int32 runIndex, ReaderConfig config)
at pwiz.ProteowizardWrapper.MsDataFileImpl..ctor(String path, Int32 sampleIndex, LockMassParameters lockmassParameters, Boolean simAsSpectra, Boolean srmAsSpectra, Boolean acceptZeroLengthSpectra, Boolean requireVendorCentroidedMS1, Boolean requireVendorCentroidedMS2, Boolean ignoreZeroIntensityPoints, Int32 preferOnlyMsLevel, Boolean combineIonMobilitySpectra, Boolean trimNativeId) in C:\proj\skyline_25_1\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 200
at pwiz.Skyline.Model.Results.OpenMsDataFileParams.OpenLocalFile(String path, Int32 sampleIndex, LockMassParameters lockMassParameters) in C:\proj\skyline_25_1\pwiz_tools\Skyline\Model\Results\OpenMsDataFileParams.cs:line 42
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_25_1\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 191
--- End of inner exception stack trace ---
 
 
Nick Shulman responded:  2025-06-23 12:15
Can you send us that file "Tris_02.raw"?
Actually, I'm not sure whether "Tris_02.raw" is a single Thermo .raw file, or is a Waters .raw folder.
If it's a Waters .raw folder, it would be best if you first packaged it up in a .zip file.

Files which are less than 50MB can be attached to these support requests.
You can always upload larger files here:
https://skyline.ms/files.url
-- Nick
 
zhijun cao responded:  2025-06-24 05:40
Hi Nick,
      I uploaded the raw file. It's a Thermo raw file acquired with DDA method.
Thanks,
Zhijun
 
Nick Shulman responded:  2025-06-24 06:50
Thank you for uploading that file.
The file seems to be fine.

Can you tell me more about the folder "'\prusntyfil01\home$"? Is that something on a network? What sort of file system is that?

It might be that this error is only happening because "Tris_02.raw" is on a network share. If you copied that file instead to a drive inside of your computer you might be able to successfully extract chromatograms.
-- Nick
 
zhijun cao responded:  2025-06-24 07:26
Hi Nick,
     Thank you for your quick response.
      Yes, '\prusntyfil01\home$" is a network drive to syc my document folder. It works when I copy raw file to local drive.
Best,
Zhijun