Reimporting the same files into previously analyzed Skyline file (From the last year) resulted in different areas.

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Reimporting the same files into previously analyzed Skyline file (From the last year) resulted in different areas. anokhina  2025-06-09 14:01
 

I have a question regarding Skyline. I am trying to incorporate more files into my skyline document. I incorporated the same files again (previously, I already analyzed them). I import the same files into previously analyzed and I see 2-fold intensity differences... I was wondering why it could be the case...
Thank you,
Vika