Dear Skyline-Team,

I hope you can help me with a question regarding a Skyline tool.

I would love to use a tool like Avant-garde to determine the integration boundaries of a large label-free PRM data set. I can apply the tool to DIA or PRM test data containing heavy peptides. Therefore, I'm wondering if it's generally possible to evaluate label-free PRM data with Avant-garde? I have tried different settings of the AvG_Params file, but none has worked so far. Is there something I should consider?

I would be super thankful to receive any tips.

Best wishes,
Terese