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Untargeted lipidomics using Skyline

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Untargeted lipidomics using Skyline nidia lauzon2  2024-12-03 09:17
 

Hi, I would like to use Skyline for untargeted lipidomics analysis. I have an Exploris240, and LipidSearch for lipids identification. I am doing AcquireX for high-throuput lipidomics. I am also using more than one internal standards for lipids class normalization.

I have never used Skyline, and I would like to know if you have a Tutorial for that.

Thank you very much
Nidia
 
 
Brian Pratt responded:  2024-12-03 11:30

The first step is to use the LipidSearch results as a transition list to tell Skyline what you're looking for. I'm afraid we don't have any specific documentation for that, but I know others do it.

Here's useful information on multiple internal standards: https://skyline.ms/wiki/home/software/Skyline/page.view?name=Surrogate Standards

This is a useful tutorial as well: https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_hi_res_metabolomics

Thanks for using the Skyline support board,

Brian Pratt