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Modifications on N and P with 13C and 15N in Peptide VINKQTPNRQIW

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Modifications on N and P with 13C and 15N in Peptide VINKQTPNRQIW teerapat rojsajjakul35372  2024-06-13 07:34
 

I have all amino acid labelled in peptide VINKQTPNRQIW with 13C and 15N. When I used Peptide Settings, modifications, N and P have the same mass, and can’t distinguish one to another in skyline and skyline doesn’t pick up the signals.
 
 
Nick Shulman responded:  2024-06-13 07:46
I do not understand your question but if you send us your Skyline document it might make more sense.
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.

Files which are less than 50MB can be attached to these support requests. You can always upload larger files here:
https://skyline.ms/files.url
-- Nick