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Adding Precursor [M-1] trace to all precursors by default

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Adding Precursor [M-1] trace to all precursors by default sean jensen  2024-04-25 21:39
 

Hi!

We use the [M-1] to qualify potential interferences and correct species identification for peptides and was wondering if anyone is able to help me enable the precursor [M-1] chromatogram trace for all precursors in a document by default. I've tried manually enabling the option for each precursor, but this is impractical to do for large analyses.

Thanks in advance!

Cheers,
Sean
 
 
Nick Shulman responded:  2024-04-25 22:33
Unfortunately there really is no other way to add all of those M-1 precursors to the document. We have talked about adding a feature to let you bulk-add things like that but for now the manual way one at a time is the only way that exists.
--Nick
 
sean jensen responded:  2024-04-28 16:12
Hi Nick,

Thank you for your swift reply!

Ah ok, I'll keep my eyes peeled for the future renditions then.

Cheers
 
emil sporre32368 responded:  2025-04-24 12:18
Hi!

Is there any news about this eventual feature? Or perhaps some other trick to make it happen?

I've some 20k peptides that I'd like to monitor the [M-1] precursor for, so the manual way seems tedious to say the least.

Best,

Emil
 
Nick Shulman responded:  2025-04-24 20:19
Unfortunately, no, there is still no way to add all of those precursors.
If you send us your Skyline document I could probably send you back a document with all of those precursors added.

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.
Files which are less than 50MB can be attached to these support requests. You can always upload larger files here:
https://skyline.ms/files.url

Going through the process of adding these precursors might give us a better idea of how to implement this as a real feature.
-- Nick