Calibration Curve | naimamuntu | 2024-03-20 21:28 | |||||||||||||||||||||||||
Hi, I am currently in the process of developing an MRM assay for 4 peptides, each with four pairs of light/ heavy. I am still learning, and I went through the tutorial. I have used a multiple-point calibration approach. I utilize light peptides as internal standard and heavy peptides that are isotopically labeled. I am wondering whether the concentration of the heavy peptide spike-in within samples must match the concentration of the constant heavy peptide used for the calibration curve. Also, what are the factors influencing the choice of concentration for the heavy peptides? Thank you so much. |
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