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Can Skyline export to Agilent QqQ method? Can you do segments？

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Can Skyline export to Agilent QqQ method? Can you do segments？ wangqingok  2023-12-07 10:00
 

Hi Skyline Team,

Can skyline export to Agilent method in a segmented manner? I could only export to Agilent method with a static SRM method but I wish to know if I want to have multiple segments in the method, such as for time 1~10 minute, do MS2 scan, and for time 10~20 minute, do a transition table, and how do do this from skyline and export? I have a lot of transitions and methods to export in this way. Many Thanks!
 
 
Nick Shulman responded:  2023-12-07 14:52
If you want to tell the mass spectrometer to collect MS2 spectra for the precursor m/z's in your document, you should use the menu item:
File > Export > Isolation List

If you want to tell the mass spectrometer to collect chromatograms for particular transitions (that is, precursor m/z's and product m/z's), you should use the menu item:
File > Export > Transition List

It sounds like you want to tell your mass spectrometer to collect some spectra and some chromatograms. You might be able to achieve what you want by exporting an Isolation List and a Transition List, and then doing something with the two .csv files that you create with those menu items.
-- Nick
 
wangqingok responded:  2023-12-07 15:06
Hi Nick,

Understood. I wish to know if there is a way to directly export transition tables into segments in an Agilent QqQ method. So far I have tried and see that I can only export all transitions into just one table without the option of separating them into multiple segments. Can you please help? Thank you!