Issue running encyclopedia

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Issue running encyclopedia joel steele28028  2023-07-14 10:07
 
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Skyline-daily
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Error
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OK More Info
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System.IO.IOException: Error occurred running process.
[03:03:40] Missing .DIA file:
[03:03:40] Encountered Fatal Error!
[03:03:40] edu.washington.gs.maccoss.encyclopedia.utils.EncyclopediaException: Can't read file C:\Users\jste0061\AppData\Local\Apps\2.0\A27R8HNY.TN2\HJZCM6HZ.ERX\skyl..tion_2e441fc3bf6adc7f_0017.0000_f621a40fd78fad56
    at edu.washington.gs.maccoss.encyclopedia.filereaders.StripeFileGenerator.innerGetFile(StripeFileGenerator.java:69)
    at edu.washington.gs.maccoss.encyclopedia.filereaders.StripeFileGenerator.getFile(StripeFileGenerator.java:40)
    at edu.washington.gs.maccoss.encyclopedia.filereaders.StripeFileGenerator.getFile(StripeFileGenerator.java:27)
    at edu.washington.gs.maccoss.encyclopedia.datastructures.AbstractSearchJobData.getDiaFileReader(AbstractSearchJobData.java:34)
    at edu.washington.gs.maccoss.encyclopedia.datastructures.QuantitativeSearchJobData.getDiaFileReader(QuantitativeSearchJobData.java:25)
    at edu.washington.gs.maccoss.encyclopedia.SearchToBLIB.getProcessedJobs(SearchToBLIB.java:625)
    at edu.washington.gs.maccoss.encyclopedia.SearchToBLIB.convert(SearchToBLIB.java:495)
    at edu.washington.gs.maccoss.encyclopedia.SearchToBLIB.convertEncyclopedia(SearchToBLIB.java:362)
    at edu.washington.gs.maccoss.encyclopedia.SearchToBLIB.main(SearchToBLIB.java:133)
    at edu.washington.gs.maccoss.encyclopedia.Encyclopedia.main(Encyclopedia.java:91)

Command-line: C:\Users\jste0061\AppData\Local\Apps\2.0\A27R8HNY.TN2\HJZCM6HZ.ERX\skyl..tion_2e441fc3bf6adc7f_0017.0000_f621a40fd78fad56\Tools\openlogic-openjdk-jre-8u342-b07-windows-64\bin\java.exe -Xmx12288M -jar "C:\Users\jste0061\AppData\Local\Apps\2.0\A27R8HNY.TN2\HJZCM6HZ.ERX\skyl..tion_2e441fc3bf6adc7f_0017.0000_f621a40fd78fad56\Tools\encyclopedia-2.12.30-executable.jar" -Duser.language=en-US -Duser.region=US -Djava.io.tmpdir=C:\Windows\Temp -v2scoring "True" -i "" -libexport -o "C:\Data_Analyses\RS Human SwissProt Jun2020-z3_nce33.elib" -f "C:\Data_Analyses\RS Human SwissProt Jun2020.fasta" -l "C:\Data_Analyses\RS Human SwissProt Jun2020-z3_nce33-prosit.dlib"
Working directory:
   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) in C:\proj\skyline_23_1\pwiz_tools\Shared\Common\SystemUtil\ProcessRunner.cs:line 180
   at pwiz.Skyline.Model.Lib.EncyclopeDiaHelpers.GenerateLibrary(String encyclopeDiaLibInputFilepath, String encyclopeDiaElibOutputFilepath, String fastaFilepath, IEnumerable`1 diaDataFiles, IProgressMonitor progressMonitor, IProgressStatus& status, EncyclopeDiaConfig config, Boolean quantLibrary) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Model\Lib\EncyclopeDiaHelpers.cs:line 629
   at pwiz.Skyline.FileUI.PeptideSearch.EncyclopeDiaSearchControl.Search(EncyclopeDiaSettings settings, CancellationTokenSource token, IProgressStatus status) in C:\proj\skyline_23_1\pwiz_tools\Skyline\FileUI\PeptideSearch\EncyclopeDiaSearchDlg.cs:line 633
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Nick Shulman responded:  2023-07-14 11:00
When the Import EncyclopeDIA Search wizard is doing its work, a lot of text gets written to the big textbox in the middle of the wizard.
Can you copy all that text and send it to us?

The error message that you have here says that the file with the extension ".DIA" which was supposed to exist could not be found.

If we could see all the text in that big textbox, it might give us some clue as to why that file did not get created.

If there isn't any good information in that textbox then you will probably need to send us your files so that we can reproduce your error. That would probably mean that you would need to send us your raw files and your FASTA files. You can zip up all those files and upload them here:
https://skyline.ms/files.url
-- Nick