Dear Skyline team,
I am trying to build a PRM method for some target proteins based on synthetic peptides I ordered. I injected a mixture of these peptides in a matrix from a different organism and could get them detected using a DDA method. I uploaded the MaxQuant files as a library and the fasta file as a background proteome, so far it looked good, but when I try to add the DDA results I get an error that "chromatogram information unavailable", and can't do any extra work.
Attached is the Skyline session and I uploaded a raw file to your sharing server.
Thank you for the support!