|Using same molecules as iRT standard and surrogate internal standards?||Will Thompson||2021-07-02|
Hi Skyline Team,
I have a use case where i would like to use the same set of stable-isotope labeled molecules both as iRT standards as well as surrogate internal standards for the purposes of quantification. If i set the molecule as an iRT standard (i.e. the little "clock" icon comes up on the molecule) then when i go to the right-click menu to set that molecule as a surrogate standard the selections are greyed out. I can't think of any practical reason why this would not be allowed. Am I doing something wrong, is there a functional reason this is the case, or is this a bug?
Representative Skyline file attached. IF you look in the document grid, you do have the surrogate standards assigned to different molecules, but this is only because i did this part before applying the iRT correction.
Help as always is appreciated.