|Question about library intensity for small molecule transition list||stefanie||2021-06-24|
I am investigating Skyline's small molecule interface for our metabolomics data processing workflow. I am new to Skyline, so I apologize if this question has been asked and answered.
I am interested in importing an assay library (or transition list) with retention times and relative fragment intensities. So far, I can successfully import a transition list with these fields: PrecursorName, PrecursorFormula, PrecursorMz, ProductMz, ProductCharge, PrecursorAdduct, PrecursorRT, PrecursorRTWindow, PrecursorCE.
What is the best way for me to import this component of our spectrum data for my metabolites to Skyline? Is there a field name that would allow me to attach relative intensities to these entries as well? I noticed the LibraryIntensity field was present in the "Skyline format.tsv" import file for Webinar 10 (proteomics) but not present in the list of column names for small molecule transition lists in a previous support ticket (2018, see link below). If I'm trying to use the wrong tool, could you suggest an appropriate alternative for me in creating a Skyline-friendly library?
Thank you for your time and your assistance,