Dear Skyline Team,
I am trying to upload an mzML file converted from a Thermo .raw file with MSConvert into a Skyline document where I have already imported a chromatogram library generated by EncyclopeDIA. I do not think the file is corrupted as I am able to use it in EncyclopeDIA without any issues.
I use Skyline (64bit) 20.1.0.155 (a0e7323e3) and MSConvert (Version: 3.0.20219-6a8ecb8e3 (developer build)).
Below is a full text of the error I am getting:
At 12:11:
Failed importing results file 'C:\Users\u525581\Desktop\Skyline EX4163G_DIA_maize_embryos_310720\20200731_EX4163C_001_01.mzML'.
[IO::HandlerBinaryDataArray] Unknown binary data type.
Inner exceptions:
Exception type: System.Exception
Error message: [IO::HandlerBinaryDataArray] Unknown binary data type.
[IO::HandlerBinaryDataArray] Unknown binary data type.
at pwiz.CLI.msdata.ChromatogramList.chromatogram(Int32 index, Boolean getBinaryData)
at pwiz.ProteowizardWrapper.MsDataFileImpl.GetChromatogram(Int32 chromIndex, String& id, Single[]& timeArray, Single[]& intensityArray) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 616
at pwiz.Skyline.Model.Results.GlobalChromatogramExtractor.GetChromatogram(Int32 index, Single[]& times, Single[]& intensities) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromDataProvider.cs:line 172
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.GetChromatogram(Int32 id, Target modifiedSequence, Color peptideColor, ChromExtra& extra, TimeIntensities& timeIntensities) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 599
at pwiz.Skyline.Model.Results.ChromData.Load(ChromDataProvider provider, Target modifiedSequence, Color peptideColor) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromData.cs:line 77
at pwiz.Skyline.Model.Results.ChromDataSet.Load(ChromDataProvider provider, Target modifiedSequence, Color peptideColor) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromDataSet.cs:line 236
at pwiz.Skyline.Model.Results.PeptideChromDataSets.Load(ChromDataProvider provider) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\PeptideChromData.cs:line 144
at pwiz.Skyline.Model.Results.ChromCacheBuilder.Read(ChromDataProvider provider) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 439
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 252
Would you be able to advice how to solve this problem, please?