Peak selection for MRM

Peak selection for MRM bart van puyvelde  2020-04-24

Hi Brendan and Skyline team,

I am currently doing some data analysis for an MRM experiment and I got a question concerning the peak selection.

I would like to force Skyline to always select the peak within a 0.1minute RT window or even smaller.
I tried to do this by changing the Full-Scan settings, but apparently it does not work.

Any suggestions to get this working? I can't share the Skyline file through the support page because it contains patient data.



Brian Pratt responded:  2020-04-24

Hi Bart,

Have a look at the precursor Explicit RTand Explicit RT Window settings available in small molecule transition list import, or the document grid for peptides.

Thanks for using the Skyline support board!

Brian Pratt