MRM | xuezhangzhi | 2020-01-02 18:57 |
Dear Skyline team I am trying to analyze hundreds of MRM dataset to use skyline, but I didn’t acquire the transitions of Decoy peptides in the MRM experiment in prior as suggested by the mprophet method. Can I still use the mProphet model for peak picking, or could you provide me some suggestions?Now, I just following the tutorial for manual evaluation. I am wondering if can I automate pick manual peak and avoid of the bias (such as ( how good RT, ppm accuracy etc.)) by any statistical model? Do you have any suggestion to automate manual peak picking with statistical model for MRM dataset using Skyline? Thanks! xuezhangzhi |
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