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Ratio to Heavy is not providing values on the calibration curve

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Ratio to Heavy is not providing values on the calibration curve marharyta vasnietsova  2026-01-19 01:32
 

Hi Team, I have been dealing with an issue where I have set the sample names to Standards, Unknowns etc, given the light concentrations for the calibration curve samples that are marked as Standards, provided the Internal Standard concentration but the calibration curve (attached) has no values.

Not all Standards samples appear on the calibration curve and still it does not provide values, I assume that the ones on it have the peaks.

Could you please help me solve this issue? Thanks in advance!
 
 
Nick Shulman responded:  2026-01-19 07:41
I think one of your standard replicates has a light:heavy peak area ratio that is infinite, and that is why the slope and intercept are displayed as NaN (i.e. "not a number").
That is, one of your external standards has a heavy peak area which is zero.
If you can figure out which replicate that is, you can right-click on the chromatogram graph for that replicate and choose "Remove Peak".

Here is another support request where someone had a calibration curve that looked like yours:
https://skyline.ms/announcements/home/support/thread.view?rowId=66510
-- Nick