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Importing DIA-NN into skyline

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Importing DIA-NN into skyline robyn eisert  2025-12-18 12:26
 

Hi,

I know that this question has been answered, but I am still having issues. I believe that to open the report-lib.parquet.skyline.speclib file in skyline I need to make sure I have the correct .tsv file with "Precursor.Id", "Q.Value" and "RT" (according to a previous post). However, I can't seem to find this file in the output folder. I'm not sure if maybe I did something wrong in DIA-NN or I'm not looking in the right place. Can you help me out?

Thank you!
Robyn
 
 
Nick Shulman responded:  2025-12-18 12:44
In the newer versions of DIA-NN, I believe the two files that Skyline needs are one whose name ends in ".speclib" and one whose name ends in ".parquet". The ".parquet" file has replaced the ".tsv" file.

If you send us all of your files we can try to figure out what is going on. You can package everything up into a .zip file and upload it here:
https://skyline.ms/files.url
-- Nick
 
robyn eisert responded:  2025-12-18 13:03
Thank you! The zip file is uploaded.