Hello there,

I am completely new to Skyline MS and am just trying to open a mzML file. I keep getting thrown an error message that I can only open skyline files, but that does not seem to be true? I should be able to open a mzML file? May someone please help me troubleshoot this?

I double checked that it was not an issue with the file (i.e. corruption) itself by trying again with a mzML file that my colleague was able to open in Skyline on their computer, and I got the same error message. I converted to mzML using ProteoWizard's msConvert with no filtering applied from the original .raw file, but that does not seem to be the main issue as the .raw file cannot be opened on Skyline either.

The version I am using was downloaded directly from the main page and is named as "Skyline (64-bit) 25.1.0.237 (519d29babc)" in the About section.

I have tried both File --> Open and File --> Import --> Document and Open File from the Start Menu.

My .mzML file is able to be viewed on ProteoWizard's SeeMS and looks as expected, but I want to open on Skyline to be able to analyze it for PFAS.

My computer (PC Dell Desktop) is running Windows 11. Processor: Intel(R) Core(TM) Ultra 7 265 (2.40 GHz). 64-bit operating system, x64-based processor.

I have attached a file of the error text I am receiving and an example .mzML file I am trying to open.

Please let me know if you can help! I am very confused.

Thanks!