Dear Skyline Support Team,

I am trying to build a quantitation workflow for intact proteins, where I wish to obtain XICs for my "naked" protein, and the protein with a glycosylation.
My protein has an average mass of 76132 Da, and the most abundant charge states are between 50-55+. Following some other helpful advice, I defined my protein as a long peptide and created a modified and nonmodified version. My issue is, that I record the data with a Q-TOF and since I do not have isotopic resolution, I need to use the average mass to get Skyline to pick the correct peaks. However, when I navigate to Settings>Transition settings>Prediction and switch 'Precursor mass' to Average I am met with the following message: "High resolution MS1 filtering requires use of monoisotopic precursor masses". Does anybody know how to select average masses for this type of analysis? Thank for your help! Version is Skyline-daily (64-bit) 25.1.1.206
Best
Soren