Dear Skyline Team,

I am experiencing an issue with the calibration curve in Skyline. It seems that the program is using peak area values instead of the total area when generating the calibration curve. As a result, the regression line is counterintuitive: the signal decreases as the concentration of the standard increases, even though the total area is actually larger for higher concentrations.

Could you please confirm whether this behavior is expected, or if there is a setting I should adjust to ensure the calibration curve is based on total area values?

Thank you very much for your support.

Best regards,