Webinar 21

Dear Skyline Users,

At last, for our second webinar of 2021 we return to the popular topic of DIA/SWATH. This next webinar revisits DIA, but by parallel accumulation serial fragmentation (diaPASEF) data collected with a Bruker timsTOF instrument. The 3-organism mix experiment is the PASEF equivalent to what we presented in our popular webinar DIA/SWATH Data Analysis in Skyline Revisited.

We also thank the 165 attendees who tuned into our last webinar Using Skyline Batch for Large Scale DIA in July. We really appreciate the energy live attendees bring to these webinars.

Webinar #21: Analysis of diaPASEF Data With Skyline

[registration closed]

When: Tuesday, December 7, 8am (Pacific Time)
- plan for 1 hour presentation + 1/2 hour Q&A

  • New! Multiple organism mix diaPASEF data set from timsTOF
  • Full-featured Import Peptide Search wizard to build a spectral library from ddaPASEF data with automatic iRT calibration
  • Data quality assessment with RT, mass error, and CV plots
  • Differential group comparison with formatted volcano plot

This webinar will include an introduction and tutorial walk-through from Brendan MacLean, Skyline Principal Developer, and a presentation from Ben Collins, a leading DIA/SWATH researcher and diaPASEF paper author at Queen's University, Belfast.

Join us, learn and help us to better meet your research needs using the latest DIA method incorporated into Skyline diaPASEF.

--Skyline Team


Brendan MacLean
(Principal Developer)

Ben Collins

Ben Collins
diaPASEF researcher

Review Webinar 20

Using Skyline Batch for Large-Scale DIA
Review slides, written tutorial and data set presented at the webinar.

Review Webinar 18

DIA/SWATH Data Analysis in Skyline Revisited
See video, presentation slides, and other files presented at the webinar.