|Sebastien Gallien Ph.D., works as a Scientist at Thermo Fisher Scientific. He received his Ph.D. in analytical chemistry from the University of Strasbourg, France, at the Bioorganic Mass Spectrometry Laboratory in 2009. In 2010, he joined the Luxembourg Clinical Proteomics Center to work with Prof. Bruno Domon as a post-doctoral fellow and then a staff scientist. His research was focused on technology and methodology developments for targeted quantitative proteomics with emphasis on clinical applications. In 2016, he joined the Precision Medicine Science Center at Thermo, where he focuses developments to improve proteomics analytical workflows, with a focus on targeted analyses that lead to clinically actionable targeted protein panels. |
Towards Turnkey Targeted Proteomics Solutions Using SureQuant Internal Standard Triggered Acquisition on Orbitrap Mass SpectrometersAn extension of HR-PRM, called SureQuant method, has recently been introduced to progress targeted proteomics. This method, implemented in the native instrument control software of Orbitrap instruments, uses spiked-in internal standards to dynamically control the acquisition process and maximize its productivity. Its ability to deliver high-density, ultra-sensitive measurements in large-scale experiments has benefited to a variety of applications (including signaling pathway monitoring, global plasma profiling, or host cell protein monitoring during biopharmaceuticals development. Read More
In addition to these analytical benefits, the technique exhibits an enhanced usability and robustness, owing to its independence from time-scheduling acquisition, and therefore high potential for automation. In order to progress further towards a turnkey solution, several informatic developments have been conducted to optimize the preparation of SureQuant method and the processing of generated data. This included new data processing functionalities implemented in Skyline, which is a key component of the optimized informatic pipeline supporting the workflow.