2018 MSACL

This is the participant's page for the 2 1/2-day Proteomics 202 Short Course at MSACL on January 21 - 23, 2018 in Palm Springs, CA.

We are look forward to seeing you in Palm Springs, but before you board your flight to southern California, there are a few pieces of information we'd like to pass along and there is some preparation you need to do prior to the course. The workshop is part lecture and part hands-on tutorial, so: 

  • Plan on bringing a PC laptop to the workshop. 
    • It is possible to use a Mac laptop, but takes considerably more preparation, since you will need to either set the Mac up for dual boot to Windows or a Windows VM (VMWare has been recommended for this in the past).
  • Install Skyline 4.1 (the latest release) on your laptop. You can download Skyline from here: Skyline 4.1 - 64 bit or from the blue button on the Skyline website homepage.
  • All other materials (aside from the laptop) will be provided after you arrive. This will include a USB flash drive with data sets that we will use in the "hands-on" sessions. If your organization/institution prohibits you from using a USB flash, please download the drive contents from this page (below).

Tutorial files

Schedule

Course Instructors

When:
Sunday, Jan 21 from 2-6pm
Monday, Jan 22 from 8-5pm (1 hr. for lunch at noon) 
Tuesday, Jan 23 from 8-noon

Where:  
All sessions are in Chino B

Additional information:
Available from the course page on the MSACL website. 

As the instructors finalize their presentations, we will post them here for future reference: 

Course Content:

Day 1

2:00 pm MacCoss - Quant Intro
3:10 pm Spencer - Proteomics Specific Considerations
4:15 pm  MacLean - Intro to Skyline
4:45 pm MacLean - Hands-on: Targeted Method Editing in Skyline

Day 2

8:00 am MacLean - Targeted Method Refinement
9:30 am Spencer - Building on Prior Knowledge
10:45 am MacLean - Hands-on: Data Analysis in Skyline
1:00 pm Pino - Calibration in Proteomics
2:00 pm MacLean - Hands-on: Calibration in Skyline
3:15 pm Pino - System Suitability and Quality Control

 Day 3

8:00 am MacCoss - Case Studies in Targeted Proteomics #1
9:00 am MacCoss - Intro to Data Independent Acquisition
10:00 am MacLean - Hands-on: DIA analysis with Skyline
11:00 am Spencer - Case Studies in Targeted Proteomics #2

Supplemental material: 

Lpino - Statistical Design

All Content in Zip file (32MB)

If you have any data you would like to discuss, please bring it with you. We will try to make time for looking at your real data.

We and all other instructors are looking forward to meeting you very soon, and engaging in a stimulating couple of days of learning and discussing targeted proteomics and ways in which Skyline can help your research.

See you all soon.

--Mike MacCoss and Brendan MacLean
Course Organizers

 

  Attached Files  
   
 Day_1_pm_02_00_MacCoss_IntroQuantProteomics.pdf
 Day_1_pm_03_10_Spencer_ProteomicsConsiderations.pdf
 Day_1_pm_04_15_MacLean_Skyline_Intro.pdf
 Day_2_am_08_00_MacLean Refinement and Data Processing.pdf
 Day_2_am_08_30_Spencer_PriorKnowledge.pdf
 Day_2_pm_03_15_Pino_SysSuitability.pdf
 Day_2_pm_01_00_Pino_Calibration.pdf
 Day_3_am_08_00_MacCoss_CaseStudies1.pdf
 Day_3_am_10_00_MacLean_DIA_DDA_Tutorial.pdf
 Day_3_am_11_00_Spencer_CaseStudies2.pdf
 LPino_StatisticalDesign.pdf
 All 2018 MSACL Presentations.zip

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