Issue 712: Acetyl 2H(3) K modification disappears on heavy en medium peptides, when library is created from Maxquant

issues
Status:resolved
Assigned To:matt.chambers42@gmail.com
Type:Defect
Area:Skyline
Priority:2
Milestone: 
Opened:2020-01-21 by evy timmerman
Changed:2020-01-24 by matt.chambers42
Resolved:2020-01-24 by matt.chambers42
Resolution:Fixed
Closed:
2020-01-21 evy timmerman
Title»Acetyl 2H(3) K modification disappears on heavy en medium peptides, when library is created from Maxquant
Assigned ToGuest»Brendan MacLean
Notify»evy timmerman
Type»Defect
Area»Skyline
Priority»2
Hello, the problem is that Acetyl 2H(3) K modification disappears on heavy en medium peptides, when i want to create a library from MSMS .txt
I only need the document peptides (pyroglu starting)

The experiment is triple silac Label:13C(6) (C-term R) and Label:13C(6) 15N(4) (C-term R) AND all lysines are modified with fixed modification of acetyl 2H3 (K), also cysteines are modified fixed carbamidomethyl on cystein

What do you advise me to do , in order to be able to get the correct library? I already made sure the names of the fixed modifications match the modification.xml from maxquant....
Do you want me to send the skyline file?

This was done in the daily version (downloaded y me this week)


Kind regards,
Evy
 
 acetylation_on_heavy_and_medium_peptide.JPG

2020-01-21 Brendan MacLean
Assigned ToBrendan MacLean»matt.chambers42@gmail.com
Matt, can you have a look at this one?

2020-01-21 matt.chambers42
Hi Evy, could you attach/upload the .sky file and msms.txt? Thanks.

2020-01-24 matt.chambers42
resolve as Fixed
Statusopen»resolved
Assigned ToGuest»matt.chambers42@gmail.com
Fixed in PR 882 (https://github.com/ProteoWizard/pwiz/pull/882)

You'll find it in the next daily release. Thanks for the report!