Issue 561: Skyline-daily loosing peak integrations

issues
Status:closed
Assigned To:Guest
Type:Defect
Area:Skyline
Priority:3
Milestone:4.1
Opened:2017-12-05 by dom.domanski
Changed:2018-03-26 by Nick Shulman
Resolved:2017-12-18 by Nick Shulman
Resolution:Fixed
Closed:2018-03-26 by Nick Shulman
2017-12-05 dom.domanski
Title»Skyline-daily loosing peak integrations
Assigned ToGuest»Brendan MacLean
Type»Defect
Area»Skyline
Priority»3
Milestone»4.1
Hello,

I noticed what appears as if Skyline-daily is "loosing integrations" or by itself selecting "Remove Peak" for some peaks that were previously automatically integrated peaks.

I'm using Skyline-daily 3.7.1.11571.
I'm using a newly created MRM file, with 6 peptides and 9 precursors, with only heavy-peptides selected.
I have imported one results file from an unscheduled analysis on a Waters Xevo TQ, of a mix of these 6 heavy peptides (90 transitions).
Peaks were correctly and automatically integrated as expected after the import. "Integrate All" is selected and most peptide peaks give a good strong signal.
However, after saving and re-opening the file 6 of the 9 precursors appear to loose the integration, i.e. no peak is selected in the chromatogram (see attached screen shots in PowerPoint).
If I choose the function Refine/Reintegrate, all peaks are correctly integrated once again, but after saving and re-opening the file the same 6 of the 9 precursors are not integrated.

I have attached the Skyline-daily file.

Thank you for your help.

Dominik
 
 RB1_CV_Opt_Grp_C_RT_check_20171129.sky
 RB1_CV_Opt_Grp_C_RT_check_20171129.sky.view
 RB1_CV_Opt_Grp_C_RT_check_20171129.skyd
 Skyline-daily resseting peak integrations to none.pptx

2017-12-09 Nick Shulman
Assigned ToBrendan MacLean»Nick Shulman

2017-12-18 Nick Shulman
resolve as Fixed
Statusopen»resolved
Assigned ToGuest»Nick Shulman
This bug will be fixed in the next update of Skyline-Daily.
(The fix did not make it into the Skyline-Daily that was released today. It will be in the next Skyline-Daily after that, which will probably be in about a week).
Skyline thought that something had changed about the libraries (nothing had changed-- that was a bug) and then Skyline was trying to reintegrate peaks before the chromatograms had been loaded, and this resulted in the peak being lost.
I have fixed this.

2018-03-26 Nick Shulman
close
Statusresolved»closed
Assigned ToNick Shulman»Guest