.mzid file not getting recognized

support
.mzid file not getting recognized bhatti rushik  2025-08-11 03:09
 

I used online Galaxy server to obtain peptide identification file (.mzid file format) from the .raw spectra file of Orbitrap.
But this is not getting recognized in Skyline.
What could be the reason and is it very frequent?

 
 
Nick Shulman responded:  2025-08-11 09:12
What do you mean "not recognized"?
Typically you would build a library from these peptide search results using "File > Import > Peptide Search" or the "Build" button on the Library tab at Settings > Peptide Settings.

Are you seeing some sort of error message?

It would be helpful if you could send us your files.

Files which are less than 50MB can be attached to these support requests.

You can always upload larger files here:
https://skyline.ms/files.url
-- Nick