**I am using Skyline for quantitation of a multiple component assay with different concentration ranges for individual calibration curves.
Using MS1 data from a DDA experiment, acquired with a Q Exactive Plus instrument.
Transition settings/Full-scan/MS1 filtering/isotope peaks included: count
Transition settings/Full-scan/MS1 filtering/Peaks: 3
I can't set set individual concentration ranges for the different compounds in the assay. If I change 1 compound, they all are modified.
I would like to have one Document Grid, in which I can vary the concentrations for each compound as needed.
How is this possible in Skyline. So far I could not find the answer in a tutorial or other document.
Kind regards,
Wim Huibers
**