Hi,

I conducted MRM HR peptide quantification using the ZenoTOF 7600 (no small molecules involved).

I'm encountering an issue when trying to import the generated WIFF file into Skyline.

I have already entered the peptides via Edit → Insert → Peptides.

In Settings → Transition Settings, I configured the following:

MS1 filtering: Count

Precursor mass analyzer: Centroided

Peaks: 3

Mass Accuracy: 10 ppm

For MS/MS filtering, I left it as None.
Among the options — None, DIA, PRM, DDA, SureQuant — which one should I select?