Limit on PTM losses

support
Limit on PTM losses Juan C. Rojas E.  2025-07-22 04:58
 

Hi all,

I am working on automating the input of large lists of custom glycans into Skyline (more on this on another post) and I encountered the following error:

"Neutral losses must be less than or equal to 5000."

More detailed error is attached.

As I understand it, it came due to having a very large glycan PTM (see attached image). Would it be possible to extend the mass limit of these neutral losses?

My current workaround is to exclude these from my workflow.

Sincerely,
Juan C.

 
 
Nick Shulman responded:  2025-07-22 07:59
Back in 2015 the maximum neutral loss mass was increased from 600 to 5000:
https://skyline.ms/announcements/home/support/thread.view?entityId=668a5ae9-8f16-1032-9a14-da2025828f32

There probably does not need to be any limit there. I will look into either removing the limit or significantly increasing it.
-- Nick