Hello. I successfully completed the Small Molecule Quantification tutorial with the example SRM data but am unable to build a calibration curve with my own PRM Orbitrap data. I would like to quantify with my product ion but I'm realizing that for PRM I need to have 'p and f' in transition settings, and 'MS level 1' in quantification molecule settings or all my integrated peaks disappear. Something else to note - my precursor ion for light and heavy are different m/z's but my product ion is the same.

Do you have any advice on the combination of molecule and transition settings for me?

I have just a few files loaded in to practice - I realize this is not great curve data. I uploaded my zip if that helps.

Thanks so much!!