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modification for non-trypsic peptide

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modification for non-trypsic peptide marie-claude menet24222  2025-02-21 02:01
 

Dear Sir,

I insert the non-tryptic peptide: QGARGFPGTPGLPGVKGHRGYP.
I want to add a variable modification (Ox), It does not work for this non-tryptic peptide.
On the other hand, on the same sheet, it works for tryptic peptides.
I use Skyline 24.1 version.

Can we consider variable modifications for non-tryptic peptides?

Best regards

MCM
 
 
Nick Shulman responded:  2025-02-21 05:28
If you want to add a modification to a particular peptide, you should right-click on the peptide in the Targets tree and choose "Modify".
If you make an adjustment to the set of peptides for a protein in the Targets tree, Skyline will no longer automatically add and remove modified peptides based on the settings.
If you would like to learn more about working with modifications in Skyline, the following webinar is usually helpful:
https://skyline.ms/Webinar10.url
-- Nick