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digestion marie-claude menet24222  2025-02-11 09:00
 

Dear Sir,

I would like to digest a protein randomly.

So ultimately obtain all possible peptides from the hydrolysis of a protein sequence.
Peptides must have between 6 and 20 amino acids.

Is it possible to achieve this with Skyline?

Best

M-Claude
 
 
Nick Shulman responded:  2025-02-11 10:22
Here is a web page which ChatGPT made for me which, given a protein sequence will give you all possible peptides of a given range of lengths:
https://proteome.gs.washington.edu/~nicksh/test/substrings.html

After you get your big list of peptides you can add them to your Skyline document using the "Edit > Insert > Peptides" menu item.
-- Nick