Small molecules: Error with calibration curve: all of the external standards are missing one or more peaks

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Small molecules: Error with calibration curve: all of the external standards are missing one or more peaks remy cordazzo  2025-02-11 01:57
 

Hello! I'm trying to make a calibration curve using your software but an error message appears during the processing : all of the external standards are missing one or more peaks.
I don't have any QC but I've entered the molecule transition on all the chromatograms. The resolved requests didn't really help.
Thanks in advance.

 
 
Nick Shulman responded:  2025-02-11 06:53
Can you send me a .sky.zip file instead of the .sky file that you attached?
In Skyline you can use the menu item:
File > Share
to create a .sky.zip file containing your Skyline document that you did attach as well other supporting files including extracted chromatograms.

That "File > Share" menu item is the best way to create a file that you can send to other people because Skyline makes sure that it contains all of the necessary files.

Files which are less than 50MB can be attached to these support requests. You can always upload larger files here:
https://skyline.ms/files.url
-- Nick
 
remy cordazzo responded:  2025-02-12 07:26
Thank you very much for the tip.
You will find the zip file following this email.