Intensity values for quantification

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Intensity values for quantification cvadival  2024-10-28 12:52
 

Hello,
We are using Skyline for quantification, but we're unable to find the intensity values; we are only getting the retention time values. Please see the attached pictures for reference. Kindly assist me to get actual intensity value for quantification.
Thanks
VC

 
 
Nick Shulman responded:  2024-10-28 15:30
The "Custom Reports" tutorial is very helpful for learning about how to export lists of numbers from Skyline:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports

The chromatogram in your screenshot does not look good at all because it has only a single point in it.
Skyline really wants to do all of its quantification from chromatogram peak areas. There probably exist other tools which are better at quantifying from individual spectra.

If you want to copy the numbers from a graph in Skyline to the Clipboard, you can right-click on the graph and choose "Copy Data". You can then paste that text into something like Microsoft Excel to see what the numbers are.
-- Nick