Drift Time Window Integration

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Drift Time Window Integration asolosky  2024-10-22 10:29
 

Hello, my name is Amie and I taught the Ion Mobility filtering for lipids tutorial during the last online Skyline course (last week). During the tutorial, we discussed how it would be helpful to be able to change the integration bounds within the drift time dimension, similar to how we are able to with chromatograms. Is this possible? Normally, we get around this by changing the resolving power window in the ion mobility transition settings, but sometimes there are peaks that fall outside the horizontal violet box that we would like to be able to include in our integrations (i.e., conformers), but only for certain targets, so I wouldn't want to change the filtering for the whole document. That is why I think being able to integrate within the drift time window just like we do for LC would also be super beneficial.

I've included an example data file where this would be helpful. The retention time of interest is 9.6 mins, but at this retention time and m/z there are multiple peaks in the drift window. I would like to be able to integrate the first two peaks at drift times 23.56 and 25.36 ms (summed), but not the peak at 33.96 ms. Can you make this possible in Skyline?

Thanks in advance for taking a look at this!

 
 
asolosky responded:  2024-10-22 10:41

https://drive.google.com/drive/folders/1Oes-fLmwFIXb-6q39u82Fza-wbC5_MdN?usp=sharing

Sorry it won't let me upload the folder! The link should work.

 
Brian Pratt responded:  2024-10-22 14:32

Hi Amie,

Thanks for the data - do you perhaps have a Skyline document that helps put the problem in context?

Best regards,

Brian Pratt

 
asolosky responded:  2024-10-23 07:24

Here ya go. Thanks!