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different heavy peptide from light peptide for quantification

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different heavy peptide from light peptide for quantification 1638393444  2024-10-21 20:06
 

Dear skyline supporter, I am doing PRM to quantify my protein A. My heavy protein B is not the same as light protein A. B and A has many similar peptides, so I want to use one peptide of heavy protein B to quantify one peptide of protein A. How can I show them together with chromatograph?
 
 
Brian Pratt responded:  2024-10-22 14:57

I think you will find what you need here:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=Surrogate Standards#:~:text=Sometimes you want to normalize,Standard Type > Surrogate Standard".

Thanks for using the Skyline support board,

Brian Pratt